NASC

NASC ID: N208

Name: methionine over-accumulation

ABRC stock number: CS208

Description: Stock is homozygous for the mto2 mutation, backcrossed to WS wild- type 3 times

Donation Date: 1992-06-01

Donated by:

Hokkaido University Derek Bartlem

Donor Number: mto2

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Wassilewskija (WS)

Segregation status: homozygous

Pedigree: Backcrossed to WS wild- type 3 times.

Segregation: Yes

Transformation method: Isolated from Feldmann T-DNA insertion library, reference: Feldmann and Marks (1997) Mol Gen Genet. 208:1-9 Note: Mutation is not tagged by T-DNA and the line does not carry a copy of the T-DNA

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	mto2-1	mto2	At4g29840

EMBL accession:

AB027151

Phenotype

In young rosettes: 1) Soluble Met over-accumulates 20-fold greater than WS wild-type 2) S-adenosylmethionine (SAM) over- accumulates 3-fold 3) Soluble Thr levels are 6% of that in wild-type. Line is also resistant to 10uM ethionine, a toxic analogue of Met

References

Bartlem, D. et al. 2000. Mutation in the threonine synthase gene results in an over-accumulation of soluble methionine in Arabidopsis. Plant Physiology 123(1):101-10.PubMed ID: 10806229.